Apparent diffusion constant of protein along DNA segments

Value 0.4 µm^2/sec Range: ±0.02 µm^2/sec
Organism Bacteria Escherichia coli
Reference Elf J, Li G, Xie XS. 2007. Probing transcription factor dynamics at the single-molecule level in a living cell. Science 316:1191–94PubMed ID17525339
Method Stroboscopic illumination-varying the time interval between two laser excitation pulses
Comments This diffusion coefficient is one order of magnitude higher than the measured in vitro 1D diffusion constant (D1) of LacI dimers bound to DNA (0.046µm^2/sec BNID 102036). Apparent diffusion attributed to the contribution from 3D diffusion in between nonspecific bindings.
Entered by Uri M
ID 102038