RNA polymerase II transcription rate

Range 18-72 nucleotides/second
Organism Various
Reference Intron Delays and Transcriptional Timing during Development, Ian A. Swinburne and Pamela A. Silver, Developmental Cell, 2008, 14 (3) p.327 middle column top paragraphPubMed ID18331713
Primary Source Darzacq X et al., In vivo dynamics of RNA polymerase II transcription. Nat Struct Mol Biol. 2007 Sep14(9):796-806. DOI: 10.1038/nsmb1280 AND Femino AM, Fay FS, Fogarty K, Singer RH. Visualization of single RNA transcripts in situ. Science. 1998 Apr 24 280(5363):585-90 AND O'Brien T, Lis JT. Rapid changes in Drosophila transcription after an instantaneous heat shock. Mol Cell Biol. 1993 Jun13(6):3456-63 AND Tennyson CN, Klamut HJ, Worton RG. The human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced. Nat Genet. 1995 Feb9(2):184-90 DOI: 10.1038/ng0295-184PubMed ID17676063, 9554849, 8497261, 7719347
Comments P.327 left column bottom paragraph: "The transcription elongation rate of RNA polymerase II across a gene determines the conversion of intron length to delay time. The first measurements of RNA polymerase II transcription rates in mammalian cells were performed in the 1970s, when the average rate of increase in length of total, metabolically labeled pre-mRNA was found to be 50–100 nucleotides per second (Sehgal et al., 1976). Since then, other studies have measured transcription speeds, by several techniques and in different conditions, at 18–72 nucleotides per second (primary sources). It is unclear exactly why this broad range of velocities has been observed. Future studies will need to examine what impact variables such as organism, cell type, developmental stage, gene structure, and the conditions under which the measurements are made have on transcription elongation rates." Organisms include Humans and Drosophila
Entered by Uri M
ID 101973