Transcription rates on different growth media

Range Table - link
Organism Bacteria Escherichia coli
Reference Vogel, U and Jensen, KF. The RNA chain elongation rate in Escherichia coli depends on the growth rate. 1994. J Bacteriol. 176(10) p.2811 table 2PubMed ID7514589
Method P.2807 left column bottom paragraph: "The experimental cultures were started by dilution of exponentially growing cultures in the same medium. Cultures were grown in shaking Erlenmeyer flasks at 37°C. The Medium was either Luria-Bertani (LB) (ref 24) containing 100µg of ampicillin per ml (doubling time, 24 min) or the A+B Salt medium of Clark And Maaloe (ref 7)."
Comments P.2808 left column bottom paragraph: "Strain MAS90 overexpresses the lac repressor from the F' laC[q1] episome." P.2811 left column 3rd paragraph: "The RNA chain growth rates, measured herein, are compiled in Table 2. It appears that the two mRNAs, i.e., infB and lacZ, were elongated at similar, but not identical, rates while the untranslated rmB-cat RNA chain was elongated nearly twice as fast." P.2811 right column top paragraph: "Condon et al. (ref 8) examined growth only on LB broth and found a transcription elongation rate of 90 nucleotides per s, in agreement with [investigators’] results (Table 2)." See BNID 101904
Entered by Phil Mongiovi
ID 101905