| Range |
0.1-1 pg
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Ginsberg SD. RNA amplification strategies for small sample populations. Methods. 2005 Nov37(3):229-37. p.229 right column bottom paragraphPubMed ID16308152
|
| Primary Source |
J.E. Kacharmina, P.B. Crino, J. Eberwine, Preparation of cDNA from single cells and subcellular regions. Methods Enzymol. 303 (1999) 3–18. AND J. Phillips, J.H. Eberwine, Antisense RNA Amplification: A Linear Amplification Method for Analyzing the mRNA Population from Single Living Cells, Methods Enzymol. Suppl. 10 (1996) 283– 288. AND J. Sambrook, D.W. Russell, Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 2001.PubMed ID10349635, 8954839
|
| Method |
(Methods mentioned in 1st 2 primary sources:) "Phenotypic characterization of cells in conjunction with single-cell mRNA analysis" AND "Patch clamp electrodes containing reverse transcriptase, dNTPS, and a poly(T) primer modified 5' with a T7 RNA polymerase promoter sequence are used to isolate the cytoplasmic contents of individual living cells." |
| Comments |
"Unfortunately, the quantity of RNA harvested from a single
cell, estimated to be approximately 0.1–1.0pg, is not
sufficient for standard RNA extraction procedures [primary sources]." |
| Entered by |
Kobi Benenson |
| ID |
101740 |