Number of rRNA genes

Value 140 genes
Organism Budding yeast Saccharomyces cerevisiae
Reference Schweizer, E. MacKechnie, C. Halverson,HO. The Redundancy of Ribosomal and Transfer RNA Genes in Saccharomyces cerevisiae. J. Mol. Biol. 1969. 40, 261-277. abstract, p.269 bottom paragraph, p.271 table 4 & p.274 2nd paragraphPubMed ID5365012
Method Abstract 1st paragraph: "DNA-RNA hybridization studies have been performed to determine the number of 4, 18 and 26 s RNA cistrons present in purified nuclear and mitochondrial DNA of Saccharomyces cerevisiae."
Comments Abstract 3rd paragraph: "Assuming a genome size of yeast nuclear DNA of 1.25×10^10 daltons, the hybridization data correspond to 140 cistrons for ribosomal RNA and to 320 to 400 cistrons for transfer RNA. With the possible exception of a minor homology between 18 and 26 s ribosomal RNA, the competition experiments indicate that there are separate cistrons for all three classes of RNA." P.269 bottom paragraph: "If one accepts a nuclear genome size in yeast of 1.25x10^10 daltons (Teuro, unpublished results), the extent of hybridization between nuclear DNA and ribosomal RNA is consistent with 140 cistrons for each 18 and 26 s rRNA (Table 4)." P.274 2nd paragraph: "Evidence is presented that S. cerevisiae nuclear DNA contains 140 cistrons for both 18 and 26 s ribosomal RNA and 320 to 400 cistrons for total transfer RNA. No homology was detected between either class of RNA and mitochondrial DNA." For ~150 genes see French et al., 2003 PMID 12588976 p.1559 left column 2nd paragraph
Entered by Phil Mongiovi
ID 101733