dGTP concentration in mid-log, LB grown E. coli, assuming a 1fL volume

Value 62 μM
Organism Bacteria Escherichia coli
Reference Wheeler LJ, Rajagopal I, Mathews CK. Stimulation of mutagenesis by proportional deoxyribonucleoside triphosphate accumulation in Escherichia coli. DNA Repair (Amst). 2005 Dec 8 4(12):1450-6 DOI: 10.1016/j.dnarep.2005.09.003 p.1452 table 3PubMed ID16207537
Method P.1452 right column top paragraph: "For [investigators'] first experiment, [they] transformed E. coli MC1061 with plasmid pPS2, a pBR322 derivative that expresses genes nrdA and nrdB of E. coli, known to encode the large and small subunits, respectively, of aerobic ribonucleotide reductase, leading to a doubling of the enzyme activity, as measured in cell-free extracts [ref 11]."
Comments P.1452 right column top paragraph: "As shown in Table 3, this caused the steady-state pools of dATP, dCTP and dTTP to increase about two-fold with respect to cells transformed by pBR322, accompanied by a smaller increase in the dGTP pool. However, these modest pool accumulations were accompanied by increases in frequency of rifampicin-resistant mutants of greater than 30-fold in three different experiments. Note that transformation of the host bacteria by pBR322 had no significant effect upon mutation frequency and only minor effects, if any, upon dNTP pools." In mid-log phase, LB grown E. coli, assuming a 1fL volume
Entered by Paul Jorgensen
ID 101429