Doubling time of haploid cell
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|Value||99 minutes Range: ±1 minutes|
|Organism||Budding yeast Saccharomyces cerevisiae|
|Reference||Talia, S. D., J. M. Skotheim, J. M. Bean, E. D. Siggia, and F. R. Cross, 2007. The effects of molecular noise and size control on variability in the budding yeast cell cycle. Nature 448:947–951. Supplementary information p. 20 table S12 Table - link PubMed ID17713537|
|Method||Researchers measured times from cytokinesis to budding (G1) and from budding to cytokinesis in haploids, diploids or tetraploids (mothers and daughters), using time-lapse fluorescence microscopy of strains expressing Myo1 tagged with green fluorescent protein (Myo1–GFP). Standard methods were used throughout strain and plasmid constructions. All strains are W303-congenic. All integrated constructs were characterized by Southern blot analysis. Cells were prepared for time-lapse microscopy as described (Bean et al. 2006 PMID 16387649). Researchers observed growth of microcolonies with fluorescence time-lapse microscopy at 30?°C using a Leica DMIRE2 inverted microscope with a Ludl motorized XY stage. Images were acquired every 3?min for cells grown in glucose and every 6?min for cells grown in glycerol/ethanol with a Hamamatsu Orca-ER camera. They used custom Visual Basic software integrated with ImagePro Plus to automate image acquisition and microscope control.|
|Comments||0.18±0.02 (Coefficient of Variation). Media and temperature dependent. Although unspecified the growth media appears to be glucose according to following sentence from p. 16 in supplementary information: "Glycerol/ethanol supports a much slower growth rate than glucose (170 min compared to 100 min doubling time)..." For doubling time of haploid mother cell from the same article see BNID 104360. For 100 min in rich medium see BNID 100270.|
|Entered by||Ben Marks|