Diameter of neutrophil

Value 8.4 µm Range: 7.3-9.7 Table - link µm
Organism Human Homo sapiens
Reference Downey GP, Doherty DE, Schwab B 3rd, Elson EL, Henson PM, Worthen GS. Retention of leukocytes in capillaries: role of cell size and deformability. J Appl Physiol. 1990 Nov69(5):1767-78. p.1771 table 1PubMed ID2272970
Method P.768 right column bottom paragraph: "The measurement of the size of isolated and purified leukocytes was done by two independent methods. The first method of leukocyte size estimation (refs 5, 34) utilized axial light extinction in the pulse width mode on a cytofluorograph (model 50H with appended 2150 Computer, Ortho Diagnostics, Westwood, MA) calibrated with standard 5-, 10-, and 15-µm latex beads (Coulter).” P.769 left column 2nd paragraph: “The second method employed a Coulter counter calibrated with latex beads of known size. Cell diameter and volume were measured using standard techniques (ref 42)."
Comments Value represents mean of coulter counter measurements of 11,000-70,000 cells from three donors. Mean neutrophil diameter measured from same samples by flow cytometer is 8.3µm, range 7.14-10.25µm. See table link for diameter of monocytes and lymphocytes. See BNID 100508, 104669
Entered by Paul Jorgensen
ID 100510