Diameter of peripheral blood lymphocytes

Value 7.3 µm Range: 6.8-7.8 µm
Organism Human Homo sapiens
Reference Kuse R, Schuster S, Schübbe H, Dix S, Hausmann K. Blood lymphocyte volumes and diameters in patients with chronic lymphocytic leukemia and normal controls. Blut. 1985 Apr50(4):243-8. SummaryPubMed ID3857080
Method Summary: "The electronic modal lymphocyte volumes of 151 patients with chronic lymphocytic leukemia (CLL) and 305 normal controls were determined by the hydrodynamically focused multi-channel Coulter TF analyser. The mean volumes of the normally distributed groups were 166±19.3 (range 126-216) µm^3 in patients with CLL and 206±14.4 (range 126-246) µm^3 in normal controls. The calculated cell diameters were 6.8 (6.2-7.4)µm and 7.3 (6.8-7.8)µm respectively." Wikipedia: A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions. When a particle flows through one of the microchannels, it results in the electrical resistance change of the liquid filled microchannel. This resistance change can be recorded as electric current or voltage pulses, which can be correlated to size, mobility, surface charge and concentration of the particles.
Comments For lymphocyte diameter of 7.02µm, range 5.81-8.14µm measured by coulter counter and 6.05µm measured by flow cytometer, range 4.92-7.25µm, both measurements of 11,000-70,000 cells from three donors, see Downey et al 1990 Table - link details in measurement method of BNID 100508,100510
Entered by Paul Jorgensen
ID 100507