Q10 (24-36°C) of TRPV4 [transient receptor potential vanilloid4] in HEK293 [Human Embryonic Kidney 293] Cells

Value 19.1 Unitless
Organism Human Homo sapiens
Reference Watanabe et al., Heat-evoked activation of TRPV4 channels in a HEK293 cell expression system and in native mouse aorta endothelial cells. J Biol Chem. 2002 Dec 6 277(49):47044-51. p.47046 figure 2E, caption to figure 2E & right column top paragraphPubMed ID12354759
Method P.47046 left column bottom paragraph:"To estimate the temperature threshold for TRPV4 activation, [investigators] have simultaneously recorded the changes in bath temperature and current (Fig. 2). At temperatures above 25 °C the TRPV4 current increased exponentially (Fig. 2, A and B)."
Comments P.47046 left column bottom paragraph:"The current plotted as a function of temperature showed hysteresis (Fig. 2 C). Ruthenium red, a selective voltage-dependent blocker of TRPV channels, rapidly inhibited the current (Fig. 2 D). Pooled data from 5 cells are shown in Fig. 2 E. The Q10 value was assessed from the slope of the log(I) versus temperature plot (see Equation 1). Obviously, at low temperatures this slope is small (Q10 = 1.6 ± 1.0) but increases strongly at temperatures above 24 °C to a Q10 value 19.1 ± 1.1. The threshold value of 24 °C was obtained from the crossover of both regression lines. Up to 43 °C no saturation was observed in these experiments. The currents activated by heat are smaller than those during stimulation with 4αPDD, indicating that further heating may recruit even more channels."
Entered by Daniel Ramot
ID 100325