Cysteine content of E. coli B/r in balanced growth at 37° C in aerobic glucose minimal medium with a mass doubling time of 40 min

Value 87 µmol/g protein Range: Table - link µmol/g protein
Organism Bacteria Escherichia coli
Reference Neidhardt F C, Umbarger H E. Modulation of chemical composition and other parameters of the cell by growth rate. In: Neidhardt F C, et al., editors Neidhardt F C, et al., editors. Escherichia coli and Salmonella: cellular and molecular biology. 2nd ed. Washington, D.C: American Society for Microbiology 1996. pp. 13–16.
Method There is 550 mg of total protein per g of dried cells Table - link (BNID 101436) With an average molecular weight of 108, there is a total of 5081 µmol of amino acid residues. The amino acid composition is based on an analysis of E. coli B/r protein by T. A. Philips, except that cysteine and tryptophan values are from ref 13, and no distinction is made between glutamate and glutamine and between aspartate and aspargine. The data have been corrected to exclude peptidoglycan (murein) amino acids. An alternative analysis is given in ref 13.
Comments Calculated for an average cell in a population of E. coli B/r in balanced growth at 37° C in aerobic glucose minimal medium with a mass doubling time of 40 min.
Entered by Ron Milo, Paul Jorgensen, Mike Springer
ID 100149