Outer Membrane thickness

Value 13 nm Range: ±1.0 nm
Organism Bacteria Escherichia coli
Reference Bayer ME. Zones of membrane adhesion in the cryofixed envelope of Escherichia coli. J Struct Biol. 1991 Dec107(3):268-80 p.271 right column bottom paragraphPubMed ID1807357
Method Electron microscopy, Abstract: "The envelopes of Escherichia coli B and E. coli K29 were examined using cryofixation and freeze substitution."
Comments P.271 right column bottom paragraph: "In contrast to this, the simple plunge-freezing in either stationary or rapidly moving cryogenic liquid (propane, Freon 13) reveals a cell envelope with a slightly wavy surface in both cell strains and a much less pronounced periplasmic space separating IM [inner membrane]and OM [outer membrane] (Fig. 3a, showing E. coli K29). In cross sections, the OM profile is of approximately equal contrast in the outer and inner leaflet, and measures 13±1.0nm." See BNID 104911, 104912, 101276, 103948
Entered by Ron Milo, Paul Jorgensen, Mike Springer
ID 100015