Enzymatic enrichment of egg-yolk phosphatidylcholine with alpha-linolenic acid

Biotechnol Lett. 2009 May;31(5):705-9. doi: 10.1007/s10529-009-9915-6. Epub 2009 Jan 23.

Abstract

alpha-Linolenic acid (ALA) was incorporated at 28% into the sn-1 position of egg-yolk phospatidylcholine using Novozyme 435 in one-step transesterification process. Using phospholipase A(2) in a two-step process gave 25% incorporation of ALA into the sn-2 position.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Egg Yolk / chemistry*
  • Enzymes, Immobilized
  • Fungal Proteins
  • Lipase / metabolism*
  • Phosphatidylcholines / isolation & purification*
  • Phosphatidylcholines / metabolism*
  • alpha-Linolenic Acid / metabolism*

Substances

  • Enzymes, Immobilized
  • Fungal Proteins
  • Phosphatidylcholines
  • alpha-Linolenic Acid
  • Novozyme 435
  • Lipase