Actin-ATP hydrolysis is a major energy drain for neurons

J Neurosci. 2003 Jan 1;23(1):1-6. doi: 10.1523/JNEUROSCI.23-01-00002.2003.

Abstract

In cultured chick ciliary neurons, when ATP synthesis is inhibited, ATP depletion is reduced approximately 50% by slowing actin filament turnover with jasplakinolide or latrunculin A. Jasplakinolide inhibits actin disassembly, and latrunculin A prevents actin assembly by sequestering actin monomers. Cytochalasin D, which allows assembly-disassembly, but only at pointed ends, is less effective in conserving ATP. Ouabain, an Na(+)-K(+)-ATPase inhibitor, and jasplakinolide both prevent approximately 50% of the ATP loss. When applied together, they completely prevent ATP loss over a period of 20 min, suggesting that filament stabilization reduces ATP consumption by decreasing actin-ATP hydrolysis directly rather than indirectly by modulating the activity of Na(+)-K(+)-ATPase, a major energy consumer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actin Cytoskeleton / drug effects
  • Actin Cytoskeleton / metabolism*
  • Actin Cytoskeleton / ultrastructure
  • Actins / metabolism*
  • Adenosine Triphosphate / metabolism*
  • Animals
  • Bridged Bicyclo Compounds, Heterocyclic / pharmacology
  • Calcium / analysis
  • Cell Hypoxia
  • Cells, Cultured
  • Chick Embryo
  • Depsipeptides*
  • Energy Metabolism
  • Enzyme Inhibitors / pharmacology
  • Hydrolysis
  • Neurons / cytology
  • Neurons / drug effects
  • Neurons / metabolism*
  • Ouabain / pharmacology
  • Peptides, Cyclic / pharmacology
  • Sodium / analysis
  • Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors
  • Thiazoles / pharmacology
  • Thiazolidines

Substances

  • Actins
  • Bridged Bicyclo Compounds, Heterocyclic
  • Depsipeptides
  • Enzyme Inhibitors
  • Peptides, Cyclic
  • Thiazoles
  • Thiazolidines
  • jasplakinolide
  • Ouabain
  • Adenosine Triphosphate
  • Sodium
  • Sodium-Potassium-Exchanging ATPase
  • latrunculin A
  • Calcium