| ID | 104312 |
| Property |
Number of polyadenylated mRNA transcripts
|
| Organism |
Budding yeast Saccharomyces cerevisiae
|
| Value |
15000
|
| Units |
Copies/cell
|
| Reference |
von der Haar T. A quantitative estimation of the global translational activity in logarithmically growing yeast cells. BMC Syst Biol. 2008 Oct 162:87 Fig. 2
|
| Reference PubMed ID |
18925958
|
| Primary Source |
Jelinsky SA, Samson LD. Global response of Saccharomyces cerevisiae to an alkylating agent. Proc Natl Acad Sci U S A. 1999 Feb 1696(4):1486-91.
|
| Primary Source PubMed ID |
9990050
|
| Method |
DNA chip technology enables simultaneous examination of how approximately 6,200 Saccharomyces cerevisiae gene transcript levels, representing the entire genome, respond to environmental change.
|
| Comments |
Although the total mass of cellular RNA cannot be calculated in the same way as proteins because non-coding mRNA regions for each gene contribute to the molecular weight but are not accurately known, the total number of mRNAs in the dataset can easily be calculated as about 12,200, with 95% confidence limits between 6,100 and 18,300 mRNAs per cell. This compares to experimental estimates of about 15,000 poly(A) tailed RNAs per cell generated experimentally [32]. In two important aspects, the curated dataset thus approaches estimates from available experimental data. See BNID 103023
|
| Entered By |
Uri M
|
| Date Added |
Jun 10, 2009 1:42 PM
|
| Date Edited |
Jan 10, 2010 7:58 AM
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| Version |
2
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| Permalink |
http://bionumbers.hms.harvard.edu//bionumber.aspx?id=104312&ver=2
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