Average replication rate of T7 bacteriophage DNA
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| Value | 220 nucleotides/sec Range: +-80 nucleotides/sec |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Lee JB, Hite RK, Hamdan SM, Xie XS, Richardson CC, van Oijen AM. DNA primase acts as a molecular brake in DNA replication. Nature. 2006 Feb 2439(7076):621-4.PubMed ID16452983 |
| Method | in vitro, single molecule measurements. The 5' end of one strand of a 48.5 kilobase (kb)-long duplex lambda phage DNA molecule is attached to the bottom surface of a glass flow cell using a biotin–streptavidin linker. The opposite 3' end bears a digoxigenin moiety and is linked to a bead that is 2.8 microm in diameter and coated with anti-digoxigenin antibody |
| Comments | These values are in agreement with Wuite, G. J. L., Smith, S. B., Young, M., Keller, D. & Bustamante, C. Single-molecule studies of the effect of template tension on T7 DNA polymerase activity. Nature (London) 404, 103-6 (2000). PubMed ID 10716452, that give polymerization rates of over 100 bases/sec for T7 DNA |
| Entered by | Uri M |
| ID | 103995 |