| Value |
6.5
min
Range: +-0.6 min
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Megerle JA, Fritz G, Gerland U, Jung K, Rädler JO. Timing and dynamics of single cell gene expression in the arabinose utilization system. Biophys J. 2008 Aug95(4):2103-15. fig. 4BPubMed ID18469087
|
| Method |
In arabinose system in e. coli. Translation was blocked by the addition of 200 µg/ml chloramphenicol, 30 min after the induction of gfp-expression with 0.2% arabinose. Fluorescence images were acquired every 3–5 min before and after inhibition.The statistics was obtained from 77 cells. |
| Comments |
All FPs, including derivatives of Aequorea victoria GFP, require a posttranslational reaction to form the fluorophore. For GFP derivatives, fluorophore maturation proceeds through two sequential steps: a relatively fast cyclization of the three amino acids at residues 65-67 followed by a slow oxidation step. This slow step dominates the maturation kinetics, and chromophore maturation effectively follows first order kinetics. |
| Entered by |
Uri M |
| ID |
102972 |