Mitochondrial pH

Range 7.98±0.07 in HeLa cells: 7.91±0.16 in rat cardiomyocytes: 7.78±0.17 in human ECV304 cells unitless
Organism Mammals
Reference H. S. Haraldsdottir, I. Thiele, & R. M. T. Fleming, Quantitative Assignment of Reaction Directionality in a Multicompartmental Human Metabolic Reconstruction, Biophysical J, February 17, 2012, supporting online material p.16/27 1st paragraphPubMed ID22768925
Primary Source 9) Llopis J, McCaffery JM, Miyawaki A, Farquhar MG, Tsien RY. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc Natl Acad Sci U S A. 1998 Jun 9 95(12):6803-8. p.6805 right column 2nd paragraph (10) Porcelli AM, Ghelli A, Zanna C, Pinton P, Rizzuto R, Rugolo M. pH difference across the outer mitochondrial membrane measured with a green fluorescent protein mutant. Biochem Biophys Res Commun. 2005 Jan 28 326(4):799-804.PubMed ID9618493, 15607740
Method EYFP-mit was targeted to the mitochondrial matrix by fusing it with a short peptide from a mitochondria specifi c protein.
Comments Mitochondrial pH was found to be 7.98 ± 0.07 in HeLa cells and 7.91 ± 0.16 in cardiomyocytes (primary source 9). Using the same method Porcelli et al. measured a pH of 7.78 ± 0.17 in ECV304 cells (human umbilical vein endothelial cells, primary source 10).
Entered by Uri M
ID 108124