Number of Env and Gag proteins per virion

Range Env 7-10: Gag ≈2,400 Copies/virion
Organism HIV
Reference Chojnacki J et al., Envelope glycoprotein mobility on HIV-1 particles depends on the virus maturation state. Nat Commun. 2017 Sep 15 8(1):545. doi: 10.1038/s41467-017-00515-6 p.2 left column top paragraph & bottom paragrahPubMed ID28916807
Primary Source [1] Zhu P, et al. Electron tomography analysis of envelope glycoprotein trimers on HIV and simian immunodeficiency virus virions. Proc. Natl Acad. Sci. 2003 100: 15812–15817. doi: 10.1073/pnas.2634931100 [2] Chojnacki J, et al. Maturation-dependent HIV-1 surface protein redistribution revealed by fluorescence nanoscopy. Science. 2012 338 :524–528. doi: 10.1126/science.1226359 [3] Carlson L-A, et al. Three-dimensional analysis of budding sites and released virus suggests a revised model for HIV-1 morphogenesis. Cell Host Microbe. 2008 4 :592–599. doi: 10.1016/j.chom.2008.10.013PubMed ID14668432, 23112332, 19064259
Method Primary source [1] electron tomography, primary source [2] fluorescence nanoscopy, primary source [3] electron tomography and scanning transmission electron microscopy
Comments P.2 left column top paragraph: "During its assembly, human immunodeficiency virus type 1 (HIV-1) incorporates 7–10 copies of the trimeric viral envelope surface glycoprotein Env [primary sources 1, 2] and  ≈ 2400 copies of the main structural protein Gag [primary source 3]—a polyprotein that initially assembles into immature virus particles (Fig. 1a)." P.2 left column bottom paragraph: "In a conventional single-point STED-FCS [Stimulated emission depletion-fluorescence correlation spectroscopy] or even FCS measurements, which usually take a few seconds, the small pool of 7–10 tagged Env molecules on an individual virus would photobleach rapidly within less than a second, and therefore this measurement modality is unsuitable."
Entered by Uri M
ID 114188