Unbalanced (calculated from the raw measurement data) uptake and secretion rates

Range Table - link
Organism Bacteria Escherichia coli
Reference Taymaz-Nikerel H, Borujeni AE, Verheijen PJ, Heijnen JJ, van Gulik WM. Genome-derived minimal metabolic models for Escherichia coli MG1655 with estimated in vivo respiratory ATP stoichiometry. Biotechnol Bioeng. 2010 Oct 1 107(2):369-81. doi: 10.1002/bit.22802. p.374 table IIIPubMed ID20506321
Method Abstract:"The unknown ATP stoichiometry parameters of the constructed E. coli network were estimated from experimental data of eight different aerobic chemostat experiments carried out with E. coli MG1655, grown at different dilution rates (0.025, 0.05, 0.1, and 0.3 h(-1)) and on different carbon substrates (glucose, glycerol, and acetate)."
Comments "The biomass specific conversion rates calculated from the primary measurements as well as the recoveries of carbon and redox are shown in Table III. It can be seen from this table that the carbon recoveries were between reasonable limits. However, for some of the glucose limited chemostats serious deviations were found for the redox balances (recoveries up to 123%). The most probable cause for this is a deviating oxygen concentration measurement in the offgas, resulting in a too high value for the oxygen uptake rate. For aerobic glucose limited growth the oxygen uptake rate is expected to be slightly lower than the carbon dioxide production rate, which was in several cases not observed in [investigators'] chemostat cultures. Deviating oxygen measurements were not used in the subsequent data reconciliation and parameter estimation procedure." Title of table:"Unbalanced (calculated from the raw measurement data) uptake and secretion rates, qi, expressed per Cmol of biomass (mmol/CmolX/h) of the steady state aerobic carbon-limited chemostat cultivations of E. coli, carried out at different dilution rates with different substrates." For conditions of experiments see table I beneath table III.
Entered by Uri M
ID 111458