Single molecule RNA polymerase II transcription rates

Value 12.8 nucleotides/sec Range: ±4.9 nucleotides/sec
Organism Bacteria Escherichia coli
Reference Adelman K, La Porta A, Santangelo TJ, Lis JT, Roberts JW, Wang MD. Single molecule analysis of RNA polymerase elongation reveals uniform kinetic behavior. Proc Natl Acad Sci U S A. 2002 Oct 15 99(21):13538-43.PubMed ID12370445
Method Optical trap of E.coli rpoA, instantaneous velocity from 3s window of data sampled at 130 Hz
Comments Broad distribution of velocities! Standard deviation is ±4.9 nts/sec
Entered by Ian Swinburne
ID 100664