Total range of protein copies per cell (75% of proteins quantified are present in range 1,000-10,000molecules/cell)

Range 0 - 1.3×10^6 molecules/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Brandon Ho et al., Comparative analysis of protein abundance studies to quantify the Saccharomyces cerevisiae proteome, bioRxiv preprint first posted online Feb. 2, 2017, doi: link p.8 left column top paragraph
Method P.7 right column bottom paragraph: "Here [investigators] provide a comprehensive view of protein abundance in yeast by normalizing and combining 19 abundance datasets, collected by mass spectrometry (Lu et al. 2007, de Godoy et al. 2008, Lee et al. 2011, Thakur et al. 2011, Peng et al. 2012, Nagaraj et al. 2012, Kulak et al. 2014, Lawless et al. 2016), GFP fluorescence flow cytometry (Newman et. al. 2006, Lee et al. 2007, Davidson et al. 2011), GFP fluorescence microscopy (Tkach et al. 2012, Breker et al. 2013, Denervaud et al. 2013, Mazumder et al. 2013, Chong et al. 2015, Yofe et al. 2016), and western blotting (Ghaemmaghami et al. 2003)."
Comments P.8 left column top paragraph: "Collectively, [investigators’] analysis suggests protein abundance in the yeast proteome ranges from zero to 1.3 x 10^6 molecules per cell. Interestingly, 75% of yeast proteins quantified are present at between 1000 and 10 000 molecules per cell, indicating that it is rare for proteins to be present at very high or very low copy numbers."
Entered by Uri M
ID 114180