| Range |
translocation time 1sec: diffusion coefficient ~0.2-0.6µm^2/sec: translocation velocity 0.65µm/sec
|
| Organism |
Human Homo sapiens |
| Reference |
Grünwald D, Singer RH, Rout M. Nuclear export dynamics of RNA-protein complexes. Nature. 2011 Jul 20 475(7356):333-41. doi: 10.1038/nature10318. p.337 right column bottom paragraph to p.338 left columnPubMed ID21776079
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| Primary Source |
Mor, A. et al. Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells. Nature Cell Biol. 12, 543–552 (2010). doi: 10.1038/ncb2056.PubMed ID20453848
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| Method |
Primary source p.543 right column bottom paragraph:"To characterize in vivo export kinetics at the single mRNP level, and to understand the relative temporal contribution of export to the total kinetics of nucleocytoplasmic translocation, [investigators] established a mammalian cell system harbouring stably integrated genes that enabled the tracking of single mRNPs from the time of transcription and during nuclear transport and export." |
| Comments |
P.337 right column bottom paragraph to p.338 left column:"This detailed picture of mRNA export complements that described previously [primary source], in which a model RNA was transiently expressed and its movement traced in the nucleoplasm and during translocation using single-molecule tracking. The translocation time was estimated to be 1 s, based on the data acquisition rate of 1-s time intervals. On the basis of statistical analysis of single-molecule tracking data, a diffusion coefficient of ~0.2–0.6 μm^2/sec was calculated, and the translocation velocity given as 0.65 μm/sec." Please note: primary source gives diffusion coefficients 1-2 orders of magnitude lower, see BNID 105651 |
| Entered by |
Uri M |
| ID |
112223 |