| Range |
0.5 - 4 kb/min
|
| Organism |
Mouse Mus musculus |
| Reference |
Jonkers I, Kwak H, Lis JT. Genome-wide dynamics of Pol II elongation and its interplay with promoter proximal pausing, chromatin, and exons. Elife. 2014 Apr 29 3: e02407. doi: 10.7554/eLife.02407. p.15 top paragraphPubMed ID24843027
|
| Method |
(Abstract:) "To dissect Pol II dynamics in mouse ES cells, [researchers] inhibited Pol II transcription at either
initiation or promoter-proximal pause escape with Triptolide or Flavopiridol, and tracked Pol II
kinetically using GRO-seq [global run-on sequencing]...Finally, tracking the progression of Pol II after drug treatment establishes Pol II elongation rates at
over 1000 genes." |
| Comments |
"[Researchers] measured elongation rates ranging from 0.5 to 4 kb/min
at ~1000 genes and within different regions of genes. Strikingly, Pol II accelerates as it transcribes
through the gene body, and a large amount of variation in elongation rates occurs between genes,
and is associated with chromatin and exon density." |
| Entered by |
Uri M |
| ID |
111273 |