| Range |
total ~90,000: active pol I complexes ~15,000: active pol II/III complexes 75,000 complexes/cell
|
| Organism |
Human Homo sapiens |
| Reference |
Jackson DA, Pombo A, Iborra F. The balance sheet for transcription: an analysis of nuclear RNA metabolism in mammalian cells. FASEB J. 2000 Feb14(2):242-54. p.244 left column 2nd paragraphPubMed ID10657981
|
| Primary Source |
[20] Jackson, D. A., Iborra, F. J., Manders, E. M. M., and Cook, P. R. (1998) Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei. Mol. Biol. Cell 9, 1523–1536 [36] Pombo, A., Jackson D. A, Hollinshead, M., Wang, Z., Roeder, R. G., and Cook, P. R. (1999) Regional specialization in human nuclei: visualization of discrete sites of transcription by RNA polymerase III. EMBO J. 18, 2241–2253PubMed ID9614191, 10205177
|
| Method |
"Most of the problems can be minimized if cells are
simply permeabilized using mild detergents, washed
to remove endogenous pools, and transcription performed
under conditions that mimic those found in
vivo (primary source 20). This preserves active polymerase complexes
and allows the number of active polymerases/cell to be determined, providing the number of
nucleotides incorporated into each RNA can be
established." |
| Comments |
"In HeLa cells, this approach [see measurement method] gives
~90,000 nascent RNA chains per cell, with ~15,000
active pol I and 75,000 active pol II/III complexes,
~10% of which are pol III (primary Sources)." |
| Entered by |
Uri M |
| ID |
111166 |