Comparison of the macromolecular composition of E. coli B/r growing in glucose minimal medium

Range Table - link
Organism Bacteria Escherichia coli
Reference Brunschede H, Dove TL, Bremer H. Establishment of exponential growth after a nutritional shift-up in Escherichia coli B/r: accumulation of deoxyribonucleic acid, ribonucleic acid, and protein. J Bacteriol. 1977 Feb129(2):1020-33. p.1030 table 3PubMed ID320174
Method For RNA and protein determination, 5-ml samples of culture were precipitated with 1 ml of 3 M trichloroacetic acid at 0°C.
Comments See notes beneath table. The ratios of the amounts, RNA/DNA, RNA/protein, and protein/ DNA for cultures of E. coli B/r determined here nearly agree with those estimated previously (Table 3), although entirely different methods were used (see reference 13: measurement of the differential rate of synthesis of ribosomal protein for the RNA/protein ratio and [14C]uracil labeling for the RNA/DNA ratio).
Entered by Uri M
ID 108107