| Method |
Enzyme concentrations were roughly guessed from protein molecule numbers in the yeast S. cerevisiae, measured in a GFP assay [Huh et al, PMID 14562095]. To convert molecule numbers into concentrations, researchers assumed a spherical cell of radius 6
µm. The protein concentration reads c= Number of molecules/(Number Avogadro ×Vcell)
M, with Avogadro's constant NA=6.022×10^23 and the
cell volume measured in litres. The range is calculated by dividing and multiplying the geometric mean by the exponent of the standard deviation of the natural logarithm, 4.7. 2480/4.7=528copies/cell. 2480×4.7=11,656 copies/cell. |