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ID104979
Property Kcat (turnover rate) of activation of isoleucine by human mitochondrial Leu tRNA Synthetase
Organism Human Homo sapiens
Value 0.07
Range +-0.004
Units Sec^-1
Reference Lue SW, Kelley SO. An aminoacyl-tRNA synthetase with a defunct editing site. Biochemistry. 2005 Mar 1 44(8):3010-6 Table link - http://tinyurl.com/ylyvahj
Reference PubMed ID 15723544
Entered By Uri M
Primary Source 
Primary Source PubMed ID
Measurement MethodCloning and Preparation of tRNA Constructs, Preparation of hs mt, WT E. coli, and E. coli T252Y LeuRS, Isolation of hs mt tRNALeu and E. coli tRNALeu Misacylated with Isoleucine, Deacylation of hs mt Ile-tRNALeu and E. coli Ile-tRNALeu, Cross-Aminoacylation and Misaminoacylation Assays,
Keywords leucine, kcat, km, aminoacylation, aminoacyl, amino acid, tRNA ligase,
CommentsKinetic parameters were determined at pH 7.5 and 37 °C. For value of 18 sec^-1 in E. coli LeuRS see BNID 104981
Date Added Oct 26, 2009 1:18 PM
Date Edited Oct 26, 2009 2:16 PM
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