BioNumber Details Page

Click search to return to the full list.
ID103720
Property Estimated HeLa cell mass
Organism Human Homo sapiens
Value 3.3
Units ng
Reference Park K, Jang J, Irimia D, Sturgis J, Lee J, Robinson JP, Toner M, Bashir R. 'Living cantilever arrays' for characterization of mass of single live cells in fluids. Lab Chip. 2008 Jul8(7):1034-41.
Reference PubMed ID 18584076
Primary Source D. A. Wolff and H. Pertoft, Separation of HeLa cells by colloidal silica density gradient centrifugation. I. Separation and partial synchrony of mitotic cells. J. Cell Biol., 1972, 55, 579–585
Primary Source PubMed ID 4571230
Method micromechanical resonant cantilever mass sensors for measurement and characterization of single cells. The resonance frequency of a cantilever is inversely proportional to the square root of the mass. Therefore, the mass of the entities attached to the cantilever can be directly calculated from the resonance frequency of the cantilever. The measured mass from the frequency response was compared with the estimated value calculated from the cell density and the cell volume. The volume of the cell was measured from the confocal microscope images by Image J (Wayne Rasband, National Institute of Health, USA), and the published value (primary ref) for the density of the cell was used.
Comments The cells in ref had volumes of 2349 µm^3 and 3857 µm^3. Value is mean mass of both cells. This gives ~3×10^8 cells/gram
Entered By Uri M
Date Added Mar 22, 2009 10:38 AM
Date Edited Sep 02, 2014 2:35 AM
Version 8
Permalink http://bionumbers.hms.harvard.edu//bionumber.aspx?id=103720&ver=8
login to add BioNumbers

Post Your Comment
Comments

Retype the characters from the picture:
Related BioNumbers:
  Measured HeLa cell mass
Human Homo sapiens
2.29 ng
  Spread surface area of HeLa cell
Human Homo sapiens
1600 µm^2
  Volume of HeLa cell
Human Homo sapiens
3700 µm^3
  Number of ATP molecules in HeLa cell
Human Homo sapiens
5.3e+9 Copies/cell
  HeLa cell volume
Human Homo sapiens
2425 µm^3