Concentration of glutathione disulfide GSSG i - Budding yeast Saccharomyces ce

ID	103548
Property	Concentration of glutathione disulfide GSSG in cytosol
Organism	Budding yeast Saccharomyces cerevisiae
Value	~4
Range
Units	uM
Reference	Østergaard H, Tachibana C, Winther JR. Monitoring disulfide bond formation in the eukaryotic cytosol. J Cell Biol. 2004 Aug 2166(3):337-45.
Reference PubMed ID	15277542
Entered By	Uri M
Primary Source
Primary Source PubMed ID
Measurement Method	Using a genetically encoded glutathione-specific redox probe the concentrations of GSH and GSSG in the yeast cytosol have been estimated
Keywords	thiolate anion, protein thiols, redox, SH, PSH, thiol–disulfide, glutathione, green fluorescent protein, glutaredoxin, redox, oxidation,
Comments	Estimates of absolute concentrations of GSH and GSSG are based on only two assumptions: (1) a cytosolic pH between 6.7 and 7.3 and (2) the absence of other catalysts than glutaredoxin 1 and 2. Although no direct evidence has been found for the latter, it seems, however, highly reasonable given the slow equilibration kinetics observed in the glr1 grx1 grx2 triple knockout. It should also be emphasized that the presence of additional redox catalysts would imply an even lower cytosolic GSSG concentration than the one estimated here. If the GSH concentration of this mutant is close to that of the wild type, as supported by whole cell measurements, we find the cytosolic GSSG concentration of the wild type to be surprisingly low, ~4 µM.
Date Added	Mar 03, 2009 11:48 AM
Date Edited	Apr 03, 2009 10:13 AM

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