mRNA half-lives of β-actin gene

Range in growth hormone 3 cell 3.70±0.76hours: in C8 cell 3.92±0.77 hours
Organism Mouse Mus musculus
Reference Takeshige K et al., Cytosolic T3-binding protein modulates dynamic alteration of T3-mediated gene expression in cells. Endocr J. 2014 61(6):561-70 p.567 table 1PubMed ID24646676
Method P.567 caption to table 1: "After treatment with 100 nM T3 [triiodothyronine] for 24 h, cells were incubated with 1 μg/mL actinomycin D and 100 nM T3 for 1, 2, 4, or 6 h followed by extraction of total RNA and reverse transcription. Quantitative PCR was performed to determine mRNA expression levels. The data relative to the values obtained from cells treated for various times were plotted. The mRNA half-life was calculated from the formula obtained by linear regression using the least-squares method. The experiments were repeated 4 times to calculate the means ± SD."
Entered by Uri M
ID 115285