Cytoplasmic G-actin concentration in most living cells

Range ~100 μM
Organism Unspecified
Reference Kiuchi T, Nagai T, Ohashi K, Mizuno K. Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension. J Cell Biol. 2011 Apr 18 193(2):365-80. doi: 10.1083/jcb.201101035. p.365 right column bottom paragraphPubMed ID21502360
Comments P.365 right column bottom paragraph:"In most living cells, the cytoplasmic G-actin concentration (~100 μM) is extraordinarily higher than the critical concentration (Cc) of actin filaments (~0.1μM) (Pollard et al., 2000 BNID 109811). This high G-actin concentration is maintained by F-actin disassembly by ADF/cofilin, and via the prevention of spontaneous G-actin nucleation by G-actin–sequestering proteins, profilin and thymosin-β4, and the blockage of F-actin polymerization by F-actin–capping proteins (Pantaloni and Carlier, 1993 Barkalow et al., 1996 Bamburg et al., 1999 Pollard et al., 2000)."
Entered by Uri M
ID 112131