Average motility levels of protrusions

Range associated with presynaptic bouton 0.59 not associated with presynaptic bouton 1.3 µm/3min
Organism Rat Rattus norvegicus
Reference Ziv NE, Smith SJ. Evidence for a role of dendritic filopodia in synaptogenesis and spine formation. Neuron. 1996 Jul17(1):91-102. P.96 right column 3rd paragraphPubMed ID8755481
Method Abstract:"Axo-dendritic synaptogenesis was examined in live hippocampal cell cultures using the fluorescent dyes DiO to label dendrites and FM 4-64 to label functional presynaptic boutons."
Comments P.96 right column 3rd paragraph: "As previously noted, dendritic protrusions associated with a presynaptic bouton were, as a rule, persistent. This does not necessarily reflect a global condition of either the dendrite or the target cell as a whole. In neurons younger than 16 DIV [days in vitro], some (but not all) neighboring dendritic protrusions not associated with presynaptic boutons displayed relatively high motility levels (see, for example Figure 7A). A quantitative comparison of the average motility levels of protrusions associated with presynaptic boutons (average motility = 0.59 μm/3 min) to those not associated with presynaptic boutons (average motility = 1.30 μm/3 min) supports this observation (6 neurons, 160 filopodia and spines, 11–15 DIV)."
Entered by Uri M
ID 112041