| Range |
Table - link
|
| Organism |
Mycobacterium tuberculosis |
| Reference |
Yamada H, Yamaguchi M, Chikamatsu K, Aono A, Mitarai S. Structome analysis of virulent Mycobacterium tuberculosis, which survives with only 700 ribosomes per 0.1 fl of cytoplasm. PLoS One. 2015 Jan 28 10(1):e0117109. doi: 10.1371/journal.pone.0117109. p.9/14 table 4PubMed ID25629354
|
| Method |
p.3 bottom paragraph:"Images obtained from negative scanning through Adobe Photoshop Elements (version 9) with CanoScan 8800F were saved as TIFF files and analyzed using ImageJ and Fiji software [refs 15, 16]. Briefly, cell length was calculated by multiplying the number by 55 nm (representing the thickness of each section). The diameter (minor and major axes), perimeter, and thickness of the plasma membrane (PM), outer membrane (OM), and cell envelope of each cell were measured as a pixel value using the line selection menu in the ImageJ/Fiji window as well as a scale bar recorded on the same negative. Measured pixel values were converted to μm or nm according to the measured pixel value of the scale bar on the corresponding negatives." |
| Comments |
p.7 3rd paragraph:"Ribosome enumeration-The number of ribosomes in the cytoplasm of each cell was also determined (Table 4 and Fig. 3). This is the first report of the direct whole-cell enumeration of bacterial ribosomes in the cytoplasm based on TEM [transmission electron microscopy] examinations of serial ultrathin sections images, as there are no previously published reports describing examinations of serial ultrathin sections of bacteria preserved by CRF [cryofixation]-RFS [rapid freeze substitution]. The average total ribosome number, average number per section, and average number per 0.1 fl of cytoplasm for the five cells examined in this study were 1,347 ± 877 (range, 49–2,122), 26.0 ± 18.6 (range, 1.4–36), and 583.2 ± 333 (range, 43.3–971), respectively." |
| Entered by |
Uri M |
| ID |
111989 |