RNA polymerase II transcription elongation rate of Hsp70 transcription unit

Range ~1.5 kb/min
Organism Fruit fly Drosophila melanogaster
Reference Yao J, Ardehali MB, Fecko CJ, Webb WW, Lis JT. Intranuclear distribution and local dynamics of RNA polymerase II during transcription activation. Mol Cell. 2007 Dec 28 28(6):978-90. p.983 left column top paragraphPubMed ID18158896
Method Abstract:"Here, [investigators] evaluate the location, movement, and transcriptional dynamics of Drosophila heat shock (HS) genes both by two-photon microscopy in live polytene nuclei and by FISH in diploid nuclei."
Comments p.982 right column bottom paragraph:"Therefore, [investigators] conclude that, during the early phase of HS [Heat Shock] gene activation, Pol II that resides mainly on Hsp70 genes is in the act of elongation, and the processes of recruitment, elongation entry, termination, and dissociation are very efficient, which is expected for the known robust transcription of the induced Hsp70 gene. [Investigators] define this transcription mode as an “efficient entry” mode. Because the Hsp70 transcription unit is about 2.5 kb, the elongation speed is measured to be ~1.5 kb/min in living cells, agreeing well with previous studies (Thummel et al., 1990 BNID 111292, O'Brien and Lis, 1993 BNID 103019 and Mason and Struhl, 2005 BNID 103016)."
Entered by Uri M
ID 111722