Hippocampal CA1 spine dimensions and correlations with synaptic (PSD) area

Range Table - link
Organism Rat Rattus norvegicus
Reference KM Harris and JK Stevens, Dendritic spines of CA 1 pyramidal cells in the rat hippocampus: serial electron microscopy with reference to their biophysical characteristics, The Journal of Neuroscience, 1 August 1989, 9(8): 2982-2997 p.2989 table 2
Method Abstract:"Serial electron microscopy and 3-D reconstructions of dendritic spines from hippocampal area CA 1 dendrites were obtained to evaluate 2 questions about relationships between spine geometry and synaptic efficacy."
Comments p.2987 right column bottom paragraph:"Quantitative evaluation of spine dimensions-Dimensions of spines, synapses, and axonal varicosities were combined for all 7 dendritic segments and are summarized in Table 2. One hundred unbranched dendritic spines were complete within these photographic series. Of these, 8 were stubby spines with no constriction in their necks, and hence they were treated as spine heads. Thus, 92 spine necks were available for analysis. Fifty-eight varicosities were reconstructed and all of their synaptic vesicles counted (from photographic series 17, 18, 21, 22, 24). The correlation (r) between each spine or axonal dimension and the PSD area was determined, and the probability of a significant difference (p < 0.05) from zero correlation was tested (Horowitz, 1974). PSD area was positively correlated with total spine volume, although the correlation with spine head volume was much greater than that with spine neck volume (Table 2, Fig. 4A)." PSD=PostSynaptic Density. See notes beneath table
Entered by Uri M
ID 111663