Diameter of negatively stained EBV capsid

Value 137.4 nm Range: ±1.85 nm
Organism Epstein-Barr virus
Reference Henson BW, Perkins EM, Cothran JE, Desai P. Self-assembly of Epstein-Barr virus capsids. J Virol. 2009 Apr83(8):3877-90. doi: 10.1128/JVI.01733-08. p.3879 right column 2nd paragraphPubMed ID19158247
Method TEM, Transmission electron microscopy
Comments "The lysates were then sedimented on sucrose gradients. A light-scattering band was visualized using reflected light (Fig. 2C EBV-ALL). This band was harvested by side puncture, and the material was examined by TEM following negative staining. Icosahedral closed capsid structures were evident, and the capsomeres made up of major capsid protein (BcLF1) were clearly visible (Fig. 2D). The diameter of negatively stained EBV capsids was calculated to be 137.4±1.85 nm (n=25)."
Entered by Uri M
ID 111424