| Comments |
"Wide-field deconvolution and CLSM [Confocal laser scanning microscopy] have long been the gold
standards in optical bioimaging, but we are now witnessing a
revolution in light microscopy that will fundamentally expand
our perception of the cell. Recently, several new technologies,
collectively termed super-resolution microscopy or nanoscopy,
have been developed that break or bypass the classical
diffraction limit and shift the optical resolution down to macromolecular
or even molecular levels (Table I). Some of these
technologies have now matured from the breadboard stage
to commercially available imaging systems, making them increasingly
attractive for broad applications and defining a new
state of the art." |