Fatty acid contents in iBMK cells

Range Table - link nmole/μl cells
Organism Mammalian tissue culture cell
Reference Fan J, Kamphorst JJ, Mathew R, Chung MK, White E, Shlomi T, Rabinowitz JD. Glutamine-driven oxidative phosphorylation is a major ATP source in transformed mammalian cells in both normoxia and hypoxia. Mol Syst Biol. 2013 Dec 3 9: 712. doi: 10.1038/msb.2013.65. Supplementary information p.8 table 2PubMed ID24301801
Method "To quantify fluxes in central metabolism, [researchers] combined three types of measurements (Figure 1A, see also Methods): (i) uptake and excretion rates of major nutrients (glucose, glutamine, and oxygen, including the fraction of oxygen consumed by oxidative phosphorylation as measured by respiratory chain inhibition) and waste products (lactate, glutamate, pyruvate, and alanine) (ii) cellular DNA, RNA, protein, and fatty acid content (Supplementary Table 2) together with cellular growth rate to determine the flux of metabolic building blocks into biomass and (iii) steady-state labeling of intracellular metabolites determined by LC-MS when cells are fed media with [U-13C]-glucose or [U-13C]- glutamine (Supplementary Figures 1–4)."
Comments iBMK=Immortalized baby mouse kidney epithelial cells
Entered by Uri M
ID 110687