Maturation time for sfGFP (super folded GFP)

Value 5.63 min Range: ±0.82 min
Organism Budding yeast Saccharomyces cerevisiae
Reference Khmelinskii A et al., Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat Biotechnol. 2012 Jun 24 30(7):708-14. doi: 10.1038/nbt.2281. Supporting Online Material p.S4 bottom paragraphPubMed ID22729030
Method "Maturation rate constants of sfGFP and mCherry were determined from the induction time course data in two steps (Supplementary Fig. 8a)."
Comments "Maturation rate constants were calculated independently for each cell. Subsequently, final maturation rate constants were determined as medians values (n=35), considering only the sub-population of cells yielding a better than median ?^2 value in model fitting. The obtained maturation half-time [is]... T=5.63±0.82min for sfGFP (median±s.e.m.) (Supplementary Fig. 8a)."
Entered by Uri M
ID 110546