Base substitution transcription error rate

Range ~4e-6 misincorporations/site
Organism Nematode Caenorhabditis elegans
Reference Gout JF, Thomas WK, Smith Z, Okamoto K, Lynch M. Large-scale detection of in vivo transcription errors. Proc Natl Acad Sci U S A. 2013 Nov 12 110(46):18584-9. doi: 10.1073/pnas.1309843110. abstract & p.18588 left column 3rd paragraphPubMed ID24167253
Method P.18584 right column bottom paragraph: "[Researchers] describe a unique method for identifying transcription errors by sequencing multiple cDNAs originating from the same mRNA molecule, using a barcoding strategy to trace back the origin of individual cDNAs." P.18588 left column 3rd paragraph: "In this study, [researchers] have described a unique cDNA library preparation technique and the associated bioinformatic analyses that allow for detection of transcription errors in RNA-seq data."
Comments
Entered by Uri M
ID 110503