Mean DNA replication rate

Value 1.6 kb/min Range: ±0.3 kb/min
Organism Budding yeast Saccharomyces cerevisiae
Reference Sekedat MD, Fenyö D, Rogers RS, Tackett AJ, Aitchison JD, Chait BT. GINS motion reveals replication fork progression is remarkably uniform throughout the yeast genome. Mol Syst Biol. 2010 6: 353. doi: 10.1038/msb.2010.8 p.5 right column 2nd paragraphPubMed ID20212525
Method "[Researchers] adopted a complementary approach for assaying replication dynamics using whole genome time-resolved chromatin immunoprecipitation combined with microarray analysis of the GINS complex, an integral member of the replication fork."
Comments "Once an active origin fires, the GINS complex moves at an almost constant rate of 1.6±0.3 kb/min. Its movement through the inter-origin regions is consistent with that of a protein complex associated with a smoothly moving replication fork. This progression rate is considerably lower and more tightly distributed than those inferred from previous genome-wide measurements assayed through nascent DNA production (BNID 110149, 110150)."
Entered by Uri M
ID 110151