| Value |
1e+6
transformants/μg autonomously replicating plasmid DNA
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Gietz RD, Schiestl RH. Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method. Nat Protoc. 2007 2(1):1-4. doi:10.1038/nprot.2007.17 p.4 "anticipated results"PubMed ID17401330
|
| Method |
"[Researchers] describe a protocol for the production of frozen competent yeast cells that can be transformed with high efficiency using the
lithium acetate/single-stranded carrier DNA/PEG method." |
| Comments |
"This protocol will yield up to 1?10^6 transformants per µg of autonomously replicating plasmid DNA with a good
transforming strain. Plasmids or DNA fragments requiring integration will be less efficient. The frozen competent yeast cells are
stable for 6 months to a year with little loss in efficiency." |
| Entered by |
Uri M |
| ID |
110139 |