Range |
~600 bp/sec
|
Organism |
Bacteria Escherichia coli |
Reference |
Reyes-Lamothe R, Possoz C, Danilova O, Sherratt DJ. Independent positioning and action of Escherichia coli replisomes in live cells. Cell. 2008 Apr 4 133(1):90-102. doi: 10.1016/j.cell.2008.01.044. p.93 right column 2nd paragraphPubMed ID18394992
|
Method |
P.90 right column bottom paragraph: "Fluorescent C-terminal fusion-protein derivatives of E. coli replisome components, expressed from their endogenous chromosomal promoters, were constructed and their phenotypes
assessed by growth, flow cytometry, and microscopy." |
Comments |
P.93 right column 2nd paragraph: "Five minutes of replication corresponds to ~180kb DNA replicated for each fork, ample time to give the observed spatial separation of two independent forks." From this one can infer a value of 600bp/s for the replication rate in vivo (180,000bp/300s). |
Entered by |
Uri M |
ID |
109251 |