Concentrations of RNA and protein in the cytoplasm in cells grown without glycine betaine (GB)

Range ~180 in cells grown at 0.1 Osm: ~330 in cells grown at 1 Osm mg/ml
Organism Bacteria Escherichia coli
Reference Cayley S, Record MT Jr. Large changes in cytoplasmic biopolymer concentration with osmolality indicate that macromolecular crowding may regulate protein-DNA interactions and growth rate in osmotically stressed Escherichia coli K-12. J Mol Recognit. 2004 Sep-Oct17(5):488-96. p.493 left column 2nd paragraphPubMed ID15362109
Primary Source Cayley S, Record MT Jr. Roles of cytoplasmic osmolytes, water, and crowding in the response of Escherichia coli to osmotic stress: biophysical basis of osmoprotection by glycine betaine. Biochemistry. 2003 Nov 4 42(43):12596-609.PubMed ID14580206
Comments "...the concentrations of RNA and protein in the cytoplasm increase from ~180 mg/ml in cells grown at 0.1 Osm to ~330 mg/ml in cells grown at 1 Osm without GB (primary source)."
Entered by Uri M
ID 108398