Diffusion coefficient of tRNA before and after aminoacylation

Range 68.4±1 before: 58±2 after aminoacylation μm^2/sec
Organism Budding yeast Saccharomyces cerevisiae
Reference Potts RO, Ford NC Jr, Fournier MJ. Changes in the solution structure of yeast phenylalanine transfer ribonucleic acid associated with aminoacylation and magnesium binding. Biochemistry. 1981 Mar 17 20(6):1653-9. p.1656 right column 2nd paragraphPubMed ID7013797
Method "The effect of aminoacylation on the structure of yeast phenylalanine tRNA was evaluated by laser light scattering."
Comments "In contrast, however, the data obtained with the aminoacylated and nonacylated species generate different slopes and intercepts in 10 mM Mg2+ Aminoacylation results in a decrease in Do20,w from (6.84±0.10)X10^-7 to (5.8±0.2)X10^-7 cm2/s and an increase in the slope from (0.10±0.02) X10^-7 to (0.22±0.06)X10^-7(cm2/s)/(mg/mL). From the slope values, the average charge of nonacylated tRNAPhc is estimated to be 10±2e-, while the average charge of the aminoacylated form is 15±2e-, corresponding to an increase in negative charge of 5±2e-. Thus, while aminoacylation of tRNAPhe results in no changes in 1 mM Mg2+, an increase in negative charge and decrease in Do20,w are observed with the 10 mM Mg2+ condition."
Entered by Uri M
ID 107935