Diffusion coefficient of transcripts in the nucleus

Range 0.03 to 0.1 μm^2/sec
Organism Eukaryotes
Reference Gorski SA, Dundr M, Misteli T. The road much traveled: trafficking in the cell nucleus. Curr Opin Cell Biol. 2006 Jun18(3):284-90 p.286 right column 2nd paragraphPubMed ID16621498
Primary Source [41] Molenaar C, et al., Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells. J Cell Biol. 2004 Apr 26 165(2):191-202. [42] Shav-Tal Y, et al. Dynamics of single mRNPs in nuclei of living cells. Science. 2004 Jun 18 304(5678):1797-800. [43] Vargas DY, et al. Mechanism of mRNA transport in the nucleus. Proc Natl Acad Sci U S A. 2005 Nov 22 102(47):17008-13.PubMed ID15117966, 15205532, 16284251
Method "...several independent labeling and imaging methods..."
Comments "Most RNA molecules are rapidly exported from the nucleus after their synthesis. The fact that newly synthesized, fully processed messenger RNAs can be detected in the cytoplasm within a few minutes after their generation inspired the notion that mRNA, rRNA and tRNA movement within the nucleus might be directed in order to increase transport efficiency. Directed, energy-dependent transport of RNAs within the cell nucleus has now largely been debunked. In pioneering studies, Politz and Pederson labeled endogenous polyadenylated RNAs by in vivo hybridization using a poly(T)-oligonucleotide and demonstrated energy-independent diffusional motion [refs 39,40]. These results have been confirmed by several independent labeling and imaging methods and a diffusion coefficient of ~0.03–0.1µm^2/s has been determined [primary sources], suggesting that energy-independent movement, probably diffusion, is sufficient to effectively transport a mRNA from its sites of transcription to the nuclear pore [ref 44] (Figure 1b)."
Entered by Uri M
ID 107613