Number of chloroplasts

Value 1 Chloroplast/cell
Organism Green algae Chlamydomonas reinhardtii
Reference Coleman AW. The fate of chloroplast DNA during cell fusion, zygote maturation and zygote germination in Chlamydomonas reinhardi as revealed by DAPI staining. Exp Cell Res. 1984 Jun152(2):528-40. p.528 abstract, 2nd paragraph and introduction, 2nd paragraphPubMed ID6539223
Primary Source [1] Ris H, Plaut W. Ultrastructure of DNA-containing areas in the chloroplast of Chlamydomonas. J Cell Biol. 1962 Jun13: 383-91. [2] Coleman AW. Visualization of chloroplast DNA with two fluorochromes. Exp Cell Res. 1978 Jun114(1):95-100. [3] Ladygin, V G, Semenova, G A & Tageeva, S V, Tsitologia 17 (1975) 115 [4] Matagne RF. Transmission of chloroplast alleles in somatic fusion products obtained from vegetative cells and/or 'Gametes' of Chlamydomonas reinhardi. Curr Genet. 1981 Apr3(1):31-6. doi: 10.1007/BF00419578. p.31 right column top paragraphPubMed ID14492436, 77789, 24189950
Comments "Chloroplast DNA in Chlamydomonas, both in vegetative cells and in zygotes, occurs as aggregates (‘nucleoids’) of variable shape and size, sometimes up to 2.5µm in their longest dimension, scattered between the thylakoids of the single large cup-shaped plastid (primary sources). They can be seen easily by fluorescence microscopy after staining with such DNA-binding fluorochromes as 4’,6 diamidino-2-phenolindole (DAPI), mithramycin or ethidium bromide (EB) (after RNase treatment)."
Entered by Uri M
ID 107030