Number of p53 transcription factor molecules per cell

Range 21,000 LB19 B-lymphoblastoid cell line: 160,000 MCF-7 breast carcinoma cell line Molecules/cell
Organism Human Homo sapiens
Reference Mark D. Biggin, Animal Transcription Networks as Highly Connected, Quantitative Continua, Developmental cell Volume 21, Issue 4, 18 October 2011, Pages 611-626 DOI 10.1016/j.devcel.2011.09.008 p.617 table 1 Table - link PubMed ID22014521
Primary Source Ma L, Wagner J, Rice JJ, Hu W, Levine AJ, Stolovitzky GA. A plausible model for the digital response of p53 to DNA damage. Proc Natl Acad Sci U S A. 2005 Oct 4 102(40):14266-71.
Method Primary source abstract: "Recent observations show that the single-cell response of p53 to ionizing radiation (IR) is "digital" in that it is the number of oscillations rather than the amplitude of p53 that shows dependence on the radiation dose. [Investigators] present a model of this phenomenon. In [their] model, double-strand break (DSB) sites induced by IR interact with a limiting pool of DNA repair proteins, forming DSB-protein complexes at DNA damage foci. The persisting complexes are sensed by ataxia telangiectasia mutated (ATM), a protein kinase that activates p53 once it is phosphorylated by DNA damage."
Comments
Entered by Uri M
ID 106910