Size of protein that can diffuse freely through an unstretched cell wall layer (murein) of Gram positive bacteria

Value 25 kDa
Organism bacteria
Reference Reith J, Mayer C. Peptidoglycan turnover and recycling in Gram-positive bacteria. Appl Microbiol Biotechnol. 2011 Jul 28 8th page left column bottom paragraphPubMed ID21796380
Primary Source Demchick P, Koch AL. The permeability of the wall fabric of Escherichia coli and Bacillus subtilis. J Bacteriol. 1996 Feb178(3):768-73.PubMed ID8550511
Method (Primary source abstract:) Actively growing cultures of Escherichia coli and Bacillus subtilis were treated with boiling sodium dodecyl sulfate solutions. The sacculi were then treated with enzymes to eliminate proteins and nucleic acids. Mathematical models were then used to interpret the experimental data.
Entered by Uri M
ID 106724